Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Embryo

Cell type

Forelimb

MeSH Description

A front limb of a quadruped. (The Random House College Dictionary, 1980)

Sample

source_name

E10.5 forelimb bud

strain background

mixed

tissue/chip rxn

2 litters of limb buds

age

E10.5 (32-35somites)

genotype

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Limb buds were dissected and dissociated prior to fixation. ChIP libraries were prepared with NEBNext Ultra II Library Prep Kit series with 15 cycles of library amplifications. Excess primers were removed from PCR reactions using AmpureXP beads (A63880) at a ratio of 0.8x beads. DNA library- NEBNext Ultra II Library Prep Kit

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

37658988

Reads aligned (%)

95.3

Duplicates removed (%)

21.0

Number of peaks

314 (qval < 1E-05)

mm9

Number of total reads

37658988

Reads aligned (%)

95.0

Duplicates removed (%)

20.9

Number of peaks

359 (qval < 1E-05)