Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

cell type

mouse embryonic stem cells

strain

C57BL/6

genotype

Mettl3_KO1

chip antibody

rabbit anti-H3K4me3 (ab8580, Abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed using Auto iDeal ChIP-seq kit for Histone (diagenode) with spike-in Chromatin (Active Motif) and spike-in Antibody (Active Motif) following the manufacturer's protocols. Library preparation was performed by using KAPA HyperPlus Kits (Kapabiosystems) according to the manufacturer's protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

30315523

Reads aligned (%)

93.6

Duplicates removed (%)

13.7

Number of peaks

27327 (qval < 1E-05)

mm9

Number of total reads

30315523

Reads aligned (%)

93.4

Duplicates removed (%)

13.8

Number of peaks

27276 (qval < 1E-05)