Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MED25

Cell type

Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
PC3-FLAG_MED25
cell line
retrovirally-infected PC3 cells
chip antibody
anti-FLAG (Sigma Life Sciences, F1804)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RIPA lysis with sonication; ChIP isolation of DNA NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
32128268
Reads aligned (%)
91.9
Duplicates removed (%)
5.6
Number of peaks
1266 (qval < 1E-05)

hg38

Number of total reads
32128268
Reads aligned (%)
94.6
Duplicates removed (%)
3.5
Number of peaks
1087 (qval < 1E-05)

Base call quality data from DBCLS SRA