Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD44hi CD69hi CD103hi T cells in lung treated with Aspergillus antigen
strain background
BALB/c
genotype/variation
Wild type
treatment
Aspergillus antigen
cell type
CD44hi CD69hi CD103hi T cells

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Total cellular RNA was extracted with TRIzol reagent (Invitrogen). And total cellular DNA was extracted in accordance with Omni-ATAC protocol(Nature Methods volume 14, pages 959–962 (2017)). For RNA-Seq, TruSeq Stranded mRNA Library Preparation Kit (Illumina) was used according to the manufacturer's protocol. For ATAC-Seq, library construction was done in accordance with Omni-ATAC protocol(Nature Methods volume 14, pages 959–962 (2017)).

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
23177752
Reads aligned (%)
76.8
Duplicates removed (%)
49.6
Number of peaks
4619 (qval < 1E-05)

mm9

Number of total reads
23177752
Reads aligned (%)
76.7
Duplicates removed (%)
49.6
Number of peaks
4589 (qval < 1E-05)

Base call quality data from DBCLS SRA