Antigen

Antigen Class

TFs and others

Antigen

NCOA1

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

Isogenic HEK293 cells, SUMOylation-defective GR (GR3KR), vehicle, NCOA1 ChIP

cell line

HEK293

cell type

human embryonal kidney cell line

stably expressing

SUMOylation-defective GR (GR3KR)

treatment

EtOH vehicle

concentration

0.01%

time

1 h

chip antibody

NCOA1 (Bethyl, A300-343A, lot # 2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with specified antibody. For ATAC-seq, 100 000 isolated nuclei were treated with 2.5 µl Nextera Tn5 Transposase from Nextera kit. For ChIP-seq, NEBNext Ultra II DNA Library Prep Kit; for ATAC-seq, Illumina Nextera DNA Library Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

27329502

Reads aligned (%)

98.3

Duplicates removed (%)

5.6

Number of peaks

8353 (qval < 1E-05)

hg19

Number of total reads

27329502

Reads aligned (%)

98.0

Duplicates removed (%)

6.3

Number of peaks

8486 (qval < 1E-05)