ChIP experiments utilised ~2x10^6 cells (per immunoprecipitation) fixed for 10 minutes at 37°C in culture media containing 2.7% (w/v) formaldehyde (Sigma-Aldrich, St Louis MO, USA). Immunoprecipitation was performed using the Chromatin Immunoprecipitation Assay Kit (Merck Millipore, Billerica MA, USA) according to the manufacturers protocol withthe included salmon sperm DNA blocked agarose beads substituted with Protein A/G PLUS-Agarose Immunoprecipitation Reagent (Santa Cruz Biotechnology). Illumina 36 bp single end sequencing was performed on 5-12 ng of ChIP DNA. 10ng DNA of MBDCap enriched DNA was prepared for Ilumina sequencing using the Illumina ChIP-Seq DNA sample prep kit (IP-102-1001) according to the manufacturer’s instructions. The library preparation was analyzed on Agilent High Sensitivity DNA 1000 Chip.