GSM3901166: TC71 SA2m#1 CTCF; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Bone
Cell type
TC-71
Primary Tissue
Bone
Tissue Diagnosis
Sarcoma Ewings
Attributes by original data submitter
Sample
source_name
Ewing sarcoma cell line (TC71)
cell line
TC71
cell type
Ewing sarcoma cell line
genotype
SA2 KO
antibody
CTCF (provided in iDeal ChIP-seq kit Diagenode)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChiP were performed following manufacturer instructions using iDeal ChIP-seq kit for transcription factors and for histones (Diagenode). For this, Ewing cell lines were fixed for 10 minutes with 1% of methanol-free formaldehyde (28908, Thermo-Scientific). Chromatin was sonicated (Bioruptor, Diagenode) for 20 cycles (30-sec on, 30-sec off) set at position “high” to generate DNA fragments with an average size around 150-300pb. The library were constructed using TruSeq ChIP library preparation kit (Illumina) according to manufacturer recommendations