Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pancreas
Cell type
Pancreatic islets
NA
NA

Attributes by original data submitter

Sample

source_name
Human pancreatic islets
treatment
Control
chip antibody
H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
14558245
Reads aligned (%)
98.5
Duplicates removed (%)
6.2
Number of peaks
11941 (qval < 1E-05)

hg19

Number of total reads
14558245
Reads aligned (%)
98.4
Duplicates removed (%)
6.3
Number of peaks
11950 (qval < 1E-05)

Base call quality data from DBCLS SRA