Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pancreas

Cell type

EndoC-BH1

NA

NA

Sample

source_name

EndoC-βH1

treatment

IFN-gamma + IL-1beta 48h

chip antibody

H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

73342745

Reads aligned (%)

99.5

Duplicates removed (%)

14.0

Number of peaks

12445 (qval < 1E-05)

hg19

Number of total reads

73342745

Reads aligned (%)

99.3

Duplicates removed (%)

14.1

Number of peaks

12382 (qval < 1E-05)