Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pancreas
Cell type
EndoC-BH1
NA
NA

Attributes by original data submitter

Sample

source_name
EndoC-βH1
treatment
IFN-gamma + IL-1beta 48h
chip antibody
H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
69134255
Reads aligned (%)
99.2
Duplicates removed (%)
24.7
Number of peaks
28455 (qval < 1E-05)

hg19

Number of total reads
69134255
Reads aligned (%)
99.0
Duplicates removed (%)
24.8
Number of peaks
28460 (qval < 1E-05)

Base call quality data from DBCLS SRA