Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pancreas
Cell type
EndoC-BH1
NA
NA

Attributes by original data submitter

Sample

source_name
EndoC-βH1
treatment
Control
chip antibody
H3K27ac (Abcam ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
18017292
Reads aligned (%)
97.0
Duplicates removed (%)
9.6
Number of peaks
5193 (qval < 1E-05)

hg19

Number of total reads
18017292
Reads aligned (%)
96.8
Duplicates removed (%)
9.7
Number of peaks
5160 (qval < 1E-05)

Base call quality data from DBCLS SRA