Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa_shControl_dox_ATAC-Seq
cell line
HeLa
cell type
cervical adenocarcinoma
cell source
ATCC#CCL-2
modification
Tet-pLKO_shControl stable cells

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATAC-seq experiments were performed as previously described (Corces,et al., 2017) with the following modification: 40,000 cells for each cell line were used to perform the transposon reaction and DNA libraries were quantified using Qubit dsDNA high sensitivity reagents (Thermo Fisher Scientific) and measured Qubit 2 fluorometer ATAC-seq libraries were performed as previously described (Corces,et al., 2017)

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
157772966
Reads aligned (%)
91.8
Duplicates removed (%)
20.7
Number of peaks
115820 (qval < 1E-05)

hg19

Number of total reads
157772966
Reads aligned (%)
91.5
Duplicates removed (%)
20.9
Number of peaks
114991 (qval < 1E-05)

Base call quality data from DBCLS SRA