Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa_shControl_ctrl_ATAC-Seq

cell line

HeLa

cell type

cervical adenocarcinoma

cell source

ATCC#CCL-2

modification

Tet-pLKO_shControl stable cells

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

ATAC-seq experiments were performed as previously described (Corces,et al., 2017) with the following modification: 40,000 cells for each cell line were used to perform the transposon reaction and DNA libraries were quantified using Qubit dsDNA high sensitivity reagents (Thermo Fisher Scientific) and measured Qubit 2 fluorometer ATAC-seq libraries were performed as previously described (Corces,et al., 2017)

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

215679839

Reads aligned (%)

90.0

Duplicates removed (%)

35.3

Number of peaks

102927 (qval < 1E-05)

hg19

Number of total reads

215679839

Reads aligned (%)

89.7

Duplicates removed (%)

35.6

Number of peaks

102157 (qval < 1E-05)