GSM3900540: SOX2 naive rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SOX2
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
Naïve ESCs (NK2 H9 line)
cell type
Human embryonic stem cells (ESCs)
cell line
Naive ESCs (NK2 H9 line)
chip antibody
SOX2 (R&D AF2018)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-Seq samples were generated essentially as described (Schoenfelder et al. 2015). Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina. Libraries were single-end sequenced on an Illumina NextSeq machine at the Babraham Institute Sequencing Facility. WA09/H9 NK2 naive and primed PSCs were kindly provided by Austin Smith (Takashima et al., 2014) with permission from WiCell. All PSCs were cultured in 5% O2, 5% CO2 at 37°C. Naive PSCs were maintained as previously described (Takashima et al., 2014) in a 1:1 mixture of DMEM/F12 and Neurobasal, 0.5x N2 supplement, 0.5x B27 supplement, 1x nonessential amino acids, 2mM L-Glutamine, 1x Penicillin/Streptomycin (all from ThermoFisher Scientific), 0.1mM β-mercaptoethanol (Sigma-Aldrich), 1 μM PD0325901, 1 μM CHIR99021, 20ng/ml human LIF (all from WT-MRC Cambridge Stem Cell Institute) and 2 μM Go6983 (PKCi; Tocris) on Matrigel–coated plates (Corning). Primed PSCs were maintained on Vitronectin-coated plates (0.5 μg/cm2; ThermoFisher Scientific) in TeSR-E8 medium (StemCell Technologies).