Antigen

Antigen Class

TFs and others

Antigen

Ezh2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

EZH2_2K

strain

C57BL/6

cell type

cultured mESC (V6.5)

cell state

Fixed by formaldehyde

chip antibody

EZH2, CST, 5246S, Lot: 7

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ESC was dissociated by 0.1% Trypsin digestion, and aliquot of cell numbers were directly used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

11874834

Reads aligned (%)

92.4

Duplicates removed (%)

80.0

Number of peaks

8456 (qval < 1E-05)

mm9

Number of total reads

11874834

Reads aligned (%)

92.3

Duplicates removed (%)

80.1

Number of peaks

8410 (qval < 1E-05)