SRX6078347
GSM3893772: H3K4me3 100 ESC ROC rep2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
H3K4me3_100_ESC_ROC
strain
C57BL/6
cell type
cultured mESC (V6.5)
cell state
Fixed by formaldehyde
chip antibody
H3K4me3, Millipore, 04-745, Lot: 2872328

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The ESC was dissociated by 0.1% Trypsin digestion, and aliquot of cell numbers were directly used for in-situ ChIP After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
32085791
Reads aligned (%)
94.8
Duplicates removed (%)
79.0
Number of peaks
11926 (qval < 1E-05)

mm9

Number of total reads
32085791
Reads aligned (%)
94.7
Duplicates removed (%)
79.1
Number of peaks
11927 (qval < 1E-05)

Base call quality data from DBCLS SRA