GSM3893587: Vc- GATA2+ECs Input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC derived endothelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Endothelial cells
cell line/strain
H1
cell type
hESCs derived Endothelial cells
antibody
none
genotype
H1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, 1×107 cells of each sample were crosslinked in 1% formaldehyde with rotation for 10 minutes at room temperature. And then these crosslinked reactions were stopped by 0.125 M glycine with rotation for 5 minutes at room temperature. and cell sample were sonicated, and then ChIPed with H3K27me3 antibodies. DNA libraries were prepared for sequencing using standard Illumina protocols.