Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MEN1

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Prostate cancer cells
cell line
LNCaP
cell type
Derived from metastatic site: Left supraclavicular lymph node.
passages
9
source tissue
androgen sensitive prostate cancer
chip antibody
rabbit anti-Menin polyclonal antibody (Bethyl, INC)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were generated using the MicroPlex Library Preparation Kit (Diagenode) following the manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
58611124
Reads aligned (%)
67.1
Duplicates removed (%)
29.7
Number of peaks
1311 (qval < 1E-05)

hg38

Number of total reads
58611124
Reads aligned (%)
69.1
Duplicates removed (%)
28.2
Number of peaks
1508 (qval < 1E-05)

Base call quality data from DBCLS SRA