Antigen

Antigen Class

TFs and others

Antigen

MEN1

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

Prostate cancer cells

cell line

LNCaP

cell type

Derived from metastatic site: Left supraclavicular lymph node.

passages

9

source tissue

androgen sensitive prostate cancer

chip antibody

rabbit anti-Menin polyclonal antibody (Bethyl, INC)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were generated using the MicroPlex Library Preparation Kit (Diagenode) following the manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

58611124

Reads aligned (%)

96.2

Duplicates removed (%)

28.2

Number of peaks

1508 (qval < 1E-05)

hg19

Number of total reads

58611124

Reads aligned (%)

95.4

Duplicates removed (%)

29.7

Number of peaks

1311 (qval < 1E-05)