Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
healthy control iPSC cell line 2242-1 with TRE-H3.1K27M pB construct, subclone A
tissue
healthy control iPSC cell line 2242-1
treatment
Day 55 eOPCs (d55)
antibody
H3K27Ac (Active Motif #39133)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using Ampure XP NEBNext Ultra II and NEBNext Multiplex Oligos

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
31128599
Reads aligned (%)
94.8
Duplicates removed (%)
16.6
Number of peaks
25388 (qval < 1E-05)

hg19

Number of total reads
31128599
Reads aligned (%)
94.4
Duplicates removed (%)
16.7
Number of peaks
25201 (qval < 1E-05)

Base call quality data from DBCLS SRA