Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
healthy control iPSC cell line 2242-1 with TRE-H3.3K27M pB construct, subclone B
tissue
healthy control iPSC cell line 2242-1
treatment
Day 55 eOPCs (d55)
antibody
H3K27Ac (Active Motif #39133)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using Ampure XP NEBNext Ultra II and NEBNext Multiplex Oligos

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
33935819
Reads aligned (%)
93.7
Duplicates removed (%)
24.4
Number of peaks
15039 (qval < 1E-05)

hg19

Number of total reads
33935819
Reads aligned (%)
93.1
Duplicates removed (%)
24.7
Number of peaks
14683 (qval < 1E-05)

Base call quality data from DBCLS SRA