Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3.3 K27M mutant

Cell type

Cell type Class
Neural
Cell type
SU-DIPG-38
NA
NA

Attributes by original data submitter

Sample

source_name
SU-DIPG38 patient-derived DIPG culture
tissue
Diffuse intrinsic pontine glioma (DIPG)
antibody
H3K27M (Millipore ABE419)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells are fixed with 1% FA. Following ChIP and washes, DNA is purified using PCI purification End-repair and A-tailing performed as described in Nagaraja 2017. Adaptor ligation and indexing with NEBNext Multiplex Oligos

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
33152366
Reads aligned (%)
97.5
Duplicates removed (%)
7.2
Number of peaks
639 (qval < 1E-05)

hg19

Number of total reads
33152366
Reads aligned (%)
97.0
Duplicates removed (%)
7.4
Number of peaks
405 (qval < 1E-05)

Base call quality data from DBCLS SRA