Antigen

Antigen Class

TFs and others

Antigen

ATF4

Cell type

Cell type Class

Blood

Cell type

KG-1a

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

KG1a

cell type

acute myeloid leukemia (AML) cell line

cell line

KG1a

chip antibody

anti-ATF4 antibody (Abcam, ab184909)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ChIP procedure was performed according to a previously described protocol (Lee et al., 2006; Ying et al., 2017), using 10mg anti-ATF4 antibody (Abcam) Enriched DNA fragments were subjected to the preparation of standard libraries for ChIP-seq, according to the manufacturer's instructions, and the resulting ChIP-seq libraries were sequenced on an Illumina Hi-seq X-Ten (Annoroad Gene Technology Co., Ltd).

Sequencing Platform

instrument_model

Illumina HiSeq X Ten

hg38

Number of total reads

18091945

Reads aligned (%)

59.8

Duplicates removed (%)

15.6

Number of peaks

437 (qval < 1E-05)

hg19

Number of total reads

18091945

Reads aligned (%)

59.1

Duplicates removed (%)

15.7

Number of peaks

217 (qval < 1E-05)