GSM3869924: Input Clone a, untreated [H3K27me3]; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse embryonic stem cells stably expressing a cRAF-ErT2 expression cassette
cell type
mouse embryonic stem cells stably expressing a cRAF-ErT2 expression cassette
treatment
untreated
strain
E14tg2a
background strain
129SV
Stage
embryonic
antibody
input
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells we fixed in 1% formaldehyde at RT for 10 minutes, nuclei extracted and chromatin sonicated using a bioruptor (10 cycles, 30s on 30s off). Cleared chromatin was incubated with anti-H3K27me3 antibody (CST 9733). Input DNA serves as negative control for antibody enrichment. Libraries were prepared using the NEB Next Ultra 2 kit.