Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293 T-Rex minus Dox

crispr status

WT

cell line

HEK293 T-Rex Flag-TFIISmut

treatment

none

antibody

RNAPII (4H8)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

147957239

Reads aligned (%)

36.8

Duplicates removed (%)

67.6

Number of peaks

4925 (qval < 1E-05)

hg19

Number of total reads

147957239

Reads aligned (%)

36.7

Duplicates removed (%)

68.2

Number of peaks

4902 (qval < 1E-05)