Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

embryonic stem cells

cell type

mouse ES cells

strain

CAST/129 F1

chip antibody

H3K27ac (ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

2 million pre-plated sorted mouse ESCs were fixed in 1% formaldehyde for 10min at RT then quenched with 2.5M glycine. ChIP was performed as described in Lesch et al 2013. Swift ChIP DNA kit, according to manufacture's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

63543750

Reads aligned (%)

93.3

Duplicates removed (%)

10.7

Number of peaks

24776 (qval < 1E-05)

mm9

Number of total reads

63543750

Reads aligned (%)

93.2

Duplicates removed (%)

10.7

Number of peaks

24830 (qval < 1E-05)