ChIP-Atlas: SRX5974127

Antigen

Cell type Class: Blood
Cell type: Th17 Cells
MeSH Description: A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: ChIP assays were performed according to the manufacturer's instructions with modifications using the ChIP-IT kit (Active Motif, USA). In brief, the Th17 cells were fixed with 1% formaldehyde, and then the crosslinked chromatin was sonicated in a 4°C water bath using a Bioruptor Pico sonicator (Diagenode) to obtain DNA fragments between 150 and 500 bp in size. For Cxxc1 ChIP-seq, 5×106 Th17 cells and 6 µg of Cxxc1 antibody were used for each sample. For H3K4me3 ChIP-seq, 3×106 Th 17 cells and 4 µg of H3K4me3 antibody were used for each sample. The immunoprecipitated DNA was purified and subjected to sequencing library preparation using a VAHTSTM Universal DNA Library Prep Kit for Illumina® V2 (Vazyme Biotech Co., Ltd) according to the manufacturer's protocol. The DNA libraries were then sequenced with an Illumina HiSeq X ten system at Veritas Genetics in Hangzhou.