Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H1

NA

NA

Sample

source_name

H1

cell type

human embryonic stem cells

chip antibody

aH3 (Abcam, 1791)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was digested by micrococcal nuclease protein-DNA complexes were isolated with aH3 antibody (Abcam, 1791). Chromatin was end-repaired, poly-adenylated, Y adaptor-ligated, and PCR-amplified.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

105413087

Reads aligned (%)

99.0

Duplicates removed (%)

20.2

Number of peaks

831 (qval < 1E-05)

hg19

Number of total reads

105413087

Reads aligned (%)

98.3

Duplicates removed (%)

21.2

Number of peaks

716 (qval < 1E-05)