Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1
cell type
human embryonic stem cells
chip antibody
aK11 (lab reagent)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was sheared by sonication and protein-DNA complexes were isolated with aK11 antibody (lab reagent). Sheared DNA was end-repaired, poly-adenylated, Y adaptor-ligated, and PCR-amplified.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
12407718
Reads aligned (%)
96.9
Duplicates removed (%)
4.5
Number of peaks
810 (qval < 1E-05)

hg19

Number of total reads
12407718
Reads aligned (%)
95.1
Duplicates removed (%)
5.7
Number of peaks
641 (qval < 1E-05)

Base call quality data from DBCLS SRA