Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Spermatocytes

MeSH Description

Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

Sample

source_name

Pachynema/Diplonema

tissue

Pachynema/Diplonema

strain

C57BL/6J

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and processed as other samples, but without antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

92876490

Reads aligned (%)

39.3

Duplicates removed (%)

22.5

Number of peaks

952 (qval < 1E-05)

mm9

Number of total reads

92876490

Reads aligned (%)

87.0

Duplicates removed (%)

21.1

Number of peaks

9801 (qval < 1E-05)