Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rec8

Cell type

Cell type Class
Gonad
Cell type
Round spermatids
NA
NA

Attributes by original data submitter

Sample

source_name
Round Spermatids
tissue
Round Spermatids
strain
C57BL/6J
chip antibody
REC8 (custom)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and REC8-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
35942530
Reads aligned (%)
84.3
Duplicates removed (%)
55.2
Number of peaks
17687 (qval < 1E-05)

mm9

Number of total reads
35942530
Reads aligned (%)
84.1
Duplicates removed (%)
55.6
Number of peaks
17658 (qval < 1E-05)

Base call quality data from DBCLS SRA