Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Muscle

Cell type

RH-4

NA

NA

Sample

source_name

RH4

antibody

H3K27ac (39133, Active Motif)

enrichment target

H3K27ac

target function

Active enhancers

treatment

HDAC3 inhibitor Merck60 1 ╡M

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted from ChIP-seq samples prepared following the Active Motif ChIP-IT High Sensitivity kit protocol. Standard Illumina barcodes were introduced during library preparation

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

27194565

Reads aligned (%)

96.7

Duplicates removed (%)

13.7

Number of peaks

51754 (qval < 1E-05)

hg19

Number of total reads

27194565

Reads aligned (%)

96.3

Duplicates removed (%)

14.2

Number of peaks

51576 (qval < 1E-05)