GSM3822032: ctrl pol2 rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEk293T
treatment
DMSO control
chip antibody
Ms mAb RNA polymerase II to CTD repeat YSPTSPS (8WG16) Abcam: ab817
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated and clarified lysate was incubated with ChIP-seq antibodies on magnetic beads to immunoprecipitate corresponding protein-DNA complexs Libraries were prepared using NEBNext Ultra II DNA library Prep Kit for Illumina E7645S with NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) E7335S