Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATA6

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC Cyt49
NA
NA

Attributes by original data submitter

Sample

source_name
cell line
cell line
CyT49
cell type
hESC
Stage
PE stage
chip antibody
GATA6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were harvested at day 5 (corresponding to gut tube endoderm (GTE)) or day 10 (coressponding to NKX6.1-positive pancreatic progenitors (PP). ChIP-IT High-Sensitivity kit (Active Motif) was used for chromatin isolation followed by immunoprecipitation with Monoclonal Rat Nkx6.1 raised in Mouse - Antibody RES310 (http://www.betacell.org/pdf/betacell-resource-310.pdf) KAPA DNA Library Preparation Kits for Illumina® (Kapa Biosystems)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
32728645
Reads aligned (%)
99.1
Duplicates removed (%)
7.9
Number of peaks
11234 (qval < 1E-05)

hg19

Number of total reads
32728645
Reads aligned (%)
98.6
Duplicates removed (%)
8.5
Number of peaks
11389 (qval < 1E-05)

Base call quality data from DBCLS SRA