Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Blood
Cell type
Pro-B cells
NA
NA

Attributes by original data submitter

Sample

source_name
sorted Cd19- proB cells
cell type
Cd19- proB cells
genotype
Setd2 KO
chip antibody
H3K36me3 Ab from Active Motif (catalogue #61101)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared using a ThruPLEX DNA-seq Kit (Rubicon Genomics) and validated using a TapeStation (Agilent Technologies) and Qubit 2.0 Flurometer (Thermo Fisher Scientific).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
37657216
Reads aligned (%)
90.3
Duplicates removed (%)
44.1
Number of peaks
694 (qval < 1E-05)

mm9

Number of total reads
37657216
Reads aligned (%)
89.9
Duplicates removed (%)
44.1
Number of peaks
787 (qval < 1E-05)

Base call quality data from DBCLS SRA