Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
R-Foxd3CKO-EtOH-H3K27ac ChIP
strain background
C57BL/6
genotype/variation
Foxd3 conditional knockout
cell type
embryonic stem cells (ESCs; R cells)
cell line of origin
Foxd3 conditional knockout C57BL/6 ESCs
treatment
Lif 2i
chip antibody
H3K27ac
chip antibody vendor
Active Motif

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using a standard ChIP protocol Library was constructed using a standard Illumina protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
50994387
Reads aligned (%)
97.2
Duplicates removed (%)
39.8
Number of peaks
36080 (qval < 1E-05)

mm9

Number of total reads
50994387
Reads aligned (%)
97.0
Duplicates removed (%)
39.9
Number of peaks
36022 (qval < 1E-05)

Base call quality data from DBCLS SRA