Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
mESCs, differentiated
NA
NA

Attributes by original data submitter

Sample

source_name
dR-H3K4me1 ChIP
cell type
day 1 ESC differentiation (dR cells)
cell line of origin
dual miRNA reporter V6.5 ESCs
treatment
-Lif -2i day 1
chip antibody
H3K4me1
chip antibody vendor
Abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted using a standard ChIP protocol Library was constructed using a standard Illumina protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
34409062
Reads aligned (%)
97.1
Duplicates removed (%)
38.7
Number of peaks
362 (qval < 1E-05)

mm9

Number of total reads
34409062
Reads aligned (%)
96.9
Duplicates removed (%)
38.7
Number of peaks
391 (qval < 1E-05)

Base call quality data from DBCLS SRA