Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Others

Cell type

Cochlear Duct

MeSH Description

A spiral tube that is firmly suspended in the bony shell-shaped part of the cochlea. This ENDOLYMPH-filled cochlear duct begins at the vestibule and makes 2.5 turns around a core of spongy bone (the modiolus) thus dividing the PERILYMPH-filled spiral canal into two channels, the SCALA VESTIBULI and the SCALA TYMPANI.

Sample

source_name

E14.5 Sox2-EGFP- cochlear duct cells

strain

B6; 129S-Sox2tm2Hoch/J (Jackson Stock No. 017592)

tissue

cochlear duct

cell type

Sox2_EGFP-

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cochlear ducts were microdissected from E12-E16 temporal treated with collagenase and dispase. Cochlear ducts were dissociated using the Worthington papain dissociation kit. Sox2-EGFP+ and Sox2-EGFP- cells were sorted by FACS. Libraries were prepared from up to 50,000 cells using the Illumina Nextera kit as described by Buenrostro et al. Curr Protoc Mol Biol. 2015.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

57146122

Reads aligned (%)

89.6

Duplicates removed (%)

5.8

Number of peaks

7630 (qval < 1E-05)

mm9

Number of total reads

57146122

Reads aligned (%)

89.5

Duplicates removed (%)

5.8

Number of peaks

7653 (qval < 1E-05)