Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCB1

Cell type

Cell type Class
Epidermis
Cell type
BJ
Primary Tissue
Skin
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
BJ hTERT ER:Ras cells
tissue
proliferating human fibroblasts
constructs
Stably expressing hTERT gene; inducible expression of ER:Ras
passage
less than 20 passages
treatment
None
antibody
SNF5 - ChIP Grade ab12167

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were double-crosslinked and nuclei were isolated. Lysates were clarified from sonicated nuclei and incubated with the corresponded antibody Antibody-DNA complexes were decrosslinked and DNA was isolated by SPRI beads. Libraries were prepared as previously described (Blecher-Gonen et al., 2013). Libraries were sequenced on the an Illumina Nextseq500 platform following the manufacturer's protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
109618215
Reads aligned (%)
174.6
Duplicates removed (%)
3.7
Number of peaks
1933 (qval < 1E-05)

hg38

Number of total reads
109618215
Reads aligned (%)
176.8
Duplicates removed (%)
3.4
Number of peaks
2782 (qval < 1E-05)

Base call quality data from DBCLS SRA