ChIP-Atlas: SRX5884797

Antigen

Cell type Class: Neural
Cell type: Glioma
MeSH Description: Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Brains were collected in PBS and tumors where microdisected, mechanically dissociated and further enzimatically digested to obtain a single cell suspension. GFP+ tumoral cells were FACS sorted, growth in culture and fixed in formaldehide and pellet to submitted for ChIP-seq analysis We completed the H3K27me3 ChIP reactions using 30 μg of mouse pediatric brain tumor chromatin and 4 μg of antibody (Active Motif, cat # 39155). The ChIP reactions also contained a drosophila chromatin spike in for the normalization of the sequencing data. We diluted a small fraction of the ChIP DNA and performed qPCR using positive control primer pairs that worked well in similar assays. For H3K27me3, the primer pair targeted to the promoter region of the active gene ACTB serves as a good negative control.