Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
T cells
NA
NA

Attributes by original data submitter

Sample

source_name
WT_Day13
cell type
P14 T-cells
genotype
WT
infection
LCMV Armstrong, LCMV-c13 and LCMV mixed c13 infection
time
day 13

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were washed and lysed to generate a crude nuclei preparation Tn5 transposase tagmentation simultaneously fragments the genome and tags the resulting DNA with Illumina sequencing adapters. DNA fragments were PCR amplified and subsequently purified using Qiagen MinElute PCR Purification Kit (Qiagen, Maryland, USA). Final library quality and quantity was analyzed and measured by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) and Life Technologies Qubit3.0 Fluorometer (Life Technologies, Carlsbad, CA), respectively.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
54232038
Reads aligned (%)
99.4
Duplicates removed (%)
0.4
Number of peaks
9139 (qval < 1E-05)

mm9

Number of total reads
54232038
Reads aligned (%)
99.4
Duplicates removed (%)
0.4
Number of peaks
9220 (qval < 1E-05)

Base call quality data from DBCLS SRA