GSM3790083: ATAC LSK BMT WT rep2; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
LSK cells
NA
NA
Attributes by original data submitter
Sample
source_name
bone marrow cells
strain
C57BL/6
tissue
bone marrow
cell type
LSK
genotype
WT
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATAC-seq experiments were performed using LSK or CMP cells obtained from WT-, SKO-, RKO-, or DKO-transplanted mice. Briefly, freshly isolated 10,000 cells were pelleted and fifty microliters of transposase mixture (25 ul of 2 x TD buffer, 2.5 ul of TDE1, 0.5 ul of 1% digitonin, and 22 ul of nuclease-free water) (FC-121-1030, Illumina; G9441) was added to the cells. After transposition reactions at 37°C for 30 min, transposed DNA was purified using QIAGEN MinElute Reaction Cleanup kit. Transposed fragments were PCR-amplified, and the resulting library was sequenced on Hiseq 2500.