Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562 cell line

cell line

K562

mutation

STAG2 R614*

genotype

STAG2 R614* +/-

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

ATAC assays using bacterial Tn5 transposase were performed as described previously (PMID: 25559105 ;PMID: 28118844). Briefly 50,000 cells/sample/replicate were washed and lysed. Transposition reactions were set up with the Illumina Nextera DNA Preparation and indexing kits (Illumina #FC-121-1030). Genomic DNA was then purified, and libraries were PCR-amplified with indexing primers and sequenced on HiSeq 2500 as 100 bp single end reads at the Otago Genomics Facility (New Zealand)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

40596584

Reads aligned (%)

57.7

Duplicates removed (%)

39.2

Number of peaks

19607 (qval < 1E-05)

hg19

Number of total reads

40596584

Reads aligned (%)

57.5

Duplicates removed (%)

39.8

Number of peaks

19476 (qval < 1E-05)