Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Embryonic fibroblast
Cell type
C3H/10T1/2
NA
NA

Attributes by original data submitter

Sample

source_name
10T1/2_WT_Tam_UNC0638_3
cell line
10T1/2
genotype/variation
WT
treatment
Tamoxifen+UNC0638
chip antibody
H3K9me3 (Abcam, ab8898)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were constructed with the NEBNext ChIP-seq library prep kit following the manufacturer's recommended protocol (New England Biolabs, Ipswich, MA, USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
40085725
Reads aligned (%)
95.7
Duplicates removed (%)
31.1
Number of peaks
704 (qval < 1E-05)

mm9

Number of total reads
40085725
Reads aligned (%)
95.2
Duplicates removed (%)
33.2
Number of peaks
706 (qval < 1E-05)

Base call quality data from DBCLS SRA