Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
cell line
A549
antibody
p53
agent
nutlin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIPseq lysates were clarified from sonicated nuclei and IPed with DO-1 anti-p53 antibody ( DO-1, Santa Cruz Biotechnology, SC-126X, Lot:G0115). RNA samples were extracted with Trizol, purified on Zymo columns, and 500ng polyA selected. DNA and mRNA libraries were prepared with standard illumina compatible protocols (NEBnext Kit)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
13332626
Reads aligned (%)
87.4
Duplicates removed (%)
10.1
Number of peaks
452 (qval < 1E-05)

hg38

Number of total reads
13332626
Reads aligned (%)
89.6
Duplicates removed (%)
8.7
Number of peaks
471 (qval < 1E-05)

Base call quality data from DBCLS SRA