Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

ATAC-seq-L234-K562-CRISPRk-15777-b

cell line

K562

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

ATAC-seq experiments were carried out following the Omni-ATAC-seq protocol as previously described (PMID: 28846090), using 50,000 K562 cells per biological replicate and two replicates per sgRNA.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

16512754

Reads aligned (%)

93.0

Duplicates removed (%)

40.4

Number of peaks

3256 (qval < 1E-05)

hg19

Number of total reads

16512754

Reads aligned (%)

92.5

Duplicates removed (%)

40.7

Number of peaks

3239 (qval < 1E-05)