Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9ac

Cell type

Cell type Class
Gonad
Cell type
UWB1.289
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Ovarian cancer cell line UWB1.289
mutation status
BRCA1-mutated
antibody
H3K9ac

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared using a kit (#17-295, Millipore, Billerica, MA) following the manufacturer's protocol. DNA libraries for H3K9ac and H3K27ac ChIPseq were prepared and sequenced at the University of Chicago Genomics Facility, Knapp Center for Biomedical Discovery (Chicago, IL).

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
44688775
Reads aligned (%)
94.9
Duplicates removed (%)
59.4
Number of peaks
32657 (qval < 1E-05)

hg19

Number of total reads
44688775
Reads aligned (%)
94.7
Duplicates removed (%)
59.7
Number of peaks
32562 (qval < 1E-05)

Base call quality data from DBCLS SRA