GSM3763541: del TRF1 Suz12 rep2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Suz12
Cell type
Cell type Class
Pluripotent stem cell
Cell type
iPS cells
NA
NA
Attributes by original data submitter
Sample
source_name
p53-/- iPS Infected by TRF1 shRNA
strain/background
C57BL/6
cell type
p53-/- iPS
shRNA
TRF1
treatment
2i
chip antibody
Suz12 (Abcam ab12073)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Reverse crosslinking was achieved by addition of 20 ml of 5M NaCl and incubation at 65ºC for 8 hours. DNA was recovered by RNase and proteinase K treatment, phenol/chloroform extraction and ethanol precipitation. 10 to 15ng of DNA per sample were used for the ChIP-seq experiment. Samples were processed through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters with "NEBNext Ultra II DNA Library Prep Kit for Illumina" from New England BioLabs (catalog # E7645). Adapter-ligated libraries were completed by limited-cycle PCR and extracted with a [single] double-sided SPRI size selection. Resulting average fragment size is 490 bp from which 120 bp correspond to adaptor sequences. Libraries were applied to an Illumina flow cell for cluster generation and sequenced on an Illumina NextSeq 500 sequencer, by following the manufacturer's protocols.