Antigen

Antigen Class

TFs and others

Antigen

CTCF

Cell type

Cell type Class

Blood

Cell type

HUDEP-2

NA

NA

Sample

source_name

Mut1_Day3_CTCF_ChIP

cell type

HUDEP-2 cell line

cell type

HUDEP-2 deleleting a CTCF motif

erythropoiesis

3 days

chip antibody

CTCF(Millipore, 07-729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ChIP assay were performed as previously described (Cheng et al, 2014, PMCID: PMC4343047) Sequencing libraries were generated using NEBNext UltraII DNA library Prep kit with homemade adaptors

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

27687529

Reads aligned (%)

98.0

Duplicates removed (%)

11.7

Number of peaks

36664 (qval < 1E-05)

hg19

Number of total reads

27687529

Reads aligned (%)

97.3

Duplicates removed (%)

12.7

Number of peaks

36609 (qval < 1E-05)