GSM3759934: 9 TLK1 siGFP-1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
HeLa
cell line
HeLa
genotype
TLK1 KO
replicate
1
sirna
siCont
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
75000 cells were resuspended in 50 μl of cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) Igepal CA-630) for nuclei preparation and treated with Nextera Tn5 Transposase (Nextera DNA Library Preparation Kit, Illumina) at 37°C for 30 min. DNA was purified using MinElute PCR Purification Kit (Qiagen). ATAC libraries were prepared using NEBNext High-Fidelity 2x PCR Master Mix (NEB) following manufacturer's instructions for 11 cycles. Amplified libraries were purified using PureLink PCR Purification Kit (Thermo Fisher Scientific).