ChIP-Atlas: SRX5803317

Antigen

Cell type Class: Epidermis
Cell type: Hair Follicle
MeSH Description: A tube-like invagination of the EPIDERMIS from which the hair shaft develops and into which SEBACEOUS GLANDS open. The hair follicle is lined by a cellular inner and outer root sheath of epidermal origin and is invested with a fibrous sheath derived from the dermis. (Stedman, 26th ed) Follicles of very long hairs extend into the subcutaneous layer of tissue under the SKIN.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Mouse hair follicle stem cells were FACS-purified from CD1 WT mice at postnatal day 21. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to a ChIPmentation protocol (Schmidl et. al., Nature Methods, 2015 ). Briefly, immunoprecitiated nucleosomes on beads were tagmented by Tagmentation enzyme from the Nextera DNA Sample Prep Kit (Illumina). Follwoing tagmentation, the beads were washed and DNA elution, reerse cross-linking, DNA purification, and PCR amplification were performed. During PCR amplification, each sample were barcoded by different adaptor primers for illumina sequencing. Sequencing libraries were pooled and sequenced on the NextSeq 500 instrument with 37/38 cycles paried end high-output mode.